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- 조회 246
- 작성자 이태화
Studies on a novel gene family encoding putative
transcriptional regulators in deepwater rice and Arabidopsis
- 일시: 2001년 3월 30일(금요일) 오후 4시
- 연사: 김 정 회 박사
MSU-DOE Plant Research Laboratory
Michigan State University
- 장소: 생명관 104호 세미나실
- 문의처: 생명과학과 이영숙 교수(☎ 279-2296)
Os-GRF1 (Oryza sativa-GROWTH-REGULATING FACTOR1)
was identified in a search for genes that are differentially
expressed in deepwater rice (Oryza sativa L.) internodes in
response to gibberellin (Van der Knaap et al., 2000). Os-GRF1
has two regions displaying general features of transcription
regulators, and contains a functional nuclear localization
signal. One of those two regions, named as QLQ domain, is
similar to a protein interaction domain of SWI2/SNF2, which
is a subunit of a chromatin-remodeling complex in yeast.
The other domain, WRC, is novel and found only in plant
proteins of unknown function. To study its role in plant
growth, Os-GRF1 was expressed in Arabidopsis. Bolting of
inflorescence stem of transformed plants was severely
inhibited, indicating that Os-GRF1 may play a regulatory
role in stem elongation.
In Arabidopsis thaliana, we have identified nine homologues
of Os-GRF1, named as GRL1 (GRF1-like) through GRL9. At-GRL
proteins show the same characteristic features, including
the highly conserved QLQ and WRC domains as does OsGRF1.
RNA gel blot and promoter::GUS fusion analyses show that
GRLs are highly expressed in the shoot tips, flowers, and
To determine the function of GRLs, we have identified T-DNA
insertional mutants for GRL1 through GRL3. However, we did
not observed a phenotype for those knock-out mutants under
different growing conditions and at different developmental
Apart from the reverse genetic approach, we are going
'forward' back again !
Van der Knaap E, Kim JH, Kende, H (2000) A novel
gibberellin-induced genes from rice and its potential
regulatory role in stem growth. Plant Physiol 122: 695-704