Zebrafish as a Tool for Functional Genomics

Zebrafish as a Tool for Functional Genomics


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- 일 시 : 2001년 9월 14일(금) 16:00-17:00


 


- 장 소 : 생명동 104호 세미나실


 


- 연 사 : 김철희 교수 (충남대학교 생물학과)


 


- Abstract


The vertebrate organizer can induce a complete body axis


when transplanted to the ventral side of a host embryo by


virtue of its distinct head and trunk inducing properties.


Wingless/Wnt antagonists secreted by the organizer have


been identified as head inducers. Their ectopic expression


can promote head formation, whereas ectopic activation of


Wnt signalling during early gastrulation blocks head formation.


These observations suggest that the ability of head inducers


to inhibit Wnt signalling during formation of anterior structures


is what distinguishes them from trunk inducers that permit the


operation of posteriorizing Wnt signals. Here we describe the


zebrafish headless (hdl) mutant and show that its severe head


defects are due to a mutation in T-cell factor-3 (Tcf3), a member


of the Tcf/Lef family. Loss of Tcf3 function in the hdl mutant reveals


that hdl represses Wnt target genes. We provide genetic evidence


that a component of the Wnt signalling pathway is essential in


vertebrate head formation and patterning.

- 문의처 : 한진관 교수 (279-2126)