Regulation of Microtubule-Kinetochore Interaction during Mitosis by NC…

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  • 2016-06-07


[Jigok Lecture Series]

▶Subject: Regulation of Microtubule-Kinetochore Interaction during Mitosis by NCAPG2 and Polo-like kinase 1 and its applications

▶Speaker: Byung Il Lee, Ph.D. (National Cancer Center Korea)

▶Date: 4:30PM/June. 9(Thu.)/2016

▶Place: Auditorium(1F), Postech Biotech Center

The early event of microtubule?kinetochore attachment is a critical stage for precise chromosome segregation. Here we report that NCAPG2, which is a component of the condensin II complex, mediates chromosome segregation through microtubule?kinetochore attachment by recruiting PLK1 to prometaphase kinetochores. NCAPG2 colocalizes with PLK1 at prometaphase kinetochores and directly interacts with the polo-box domain (PBD) of PLK1 via its highly conserved C-terminal region. In both humans and Caenorhabditis elegans, when NCAPG2 is depleted, the attachment of the spindle to the kinetochore is loosened and misoriented. This is caused by the disruption of PLK1 localization to the kinetochore and by the decreased phosphorylation of its kinetochore substrate, BubR1. In addition, the crystal structure of the PBD of PLK1, in complex with the C-terminal region of NCAPG2, 1007VLS-pT-L1011, exhibits structural conservation of PBD-phosphopeptides, suggesting that the regulation of NCAPG2 function is phosphorylation-dependent. These findings suggest that NCAPG2 plays an important role in regulating proper chromosome segregation through a functional interaction with PLK1 during mitosis. We are currently trying to develop peptide based- or small molecule- PLK1 inhibitors as anticancer agents specifically targeting NCAPG2-PLK1 interaction and briefly show our strategy and status.

Also, I’ll introduce my ongoing research on regulation of intracelluar FGF2 by Apoptosis Inhibitor 5 protein revealed by structure of API5-FGF2 complex.

▶Inquiry: Prof. Lee, Seung-Woo (279-2355)
* This seminar will be given in Korean.
please refrain from taking photos during seminars. *