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리서치하이라이트
리서치하이라이트

The application of helix fusion methods in structural biology

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  • 저널명Curr Opin Struct Biol. 2020 Feb;60:110-116
    • 담당교수Jie-Oh Lee
    • 조회53
    • 작성자최고관리자
    • 2020-07-06

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    ■ Abstract
    Methods generating fusion proteins with rigid and predictable structures have been developed in recent years. Among them, helix fusion methods that link two proteins by connecting their terminal alpha helices into a single and extended alpha helix can be particularly useful because designing fusion helices is conceptually and technically simple. These methods have been shown crucial in obtaining crystals that diffract x-rays to high resolution or attaching large and symmetrical backbone proteins to small target proteins for cryo-EM analysis. The structural rigidity of the fusion helix is crucial for these applications, and the reduction of structural ambiguity and flexibility at the fusion sites will further enhance the usefulness of this method.