Arabidopsis BAG1 Functions as a Cofactor in Hsc70-Mediated Proteasomal…
첨부파일
- 첨부된 파일이 없습니다.
- 담당교수Inhwan Hwang
- 조회1,337
- 작성자최고관리자
- 2017-10-17
관련링크
본문
Eliminating misfolded or mistargeted proteins is crucial for cell
viability because these proteins accumulate as non-specific
aggregates, which can be toxic to the cell (Lee et al., 2009;
Sroka et al., 2009). Previously, we have shown that in ppi2
(plastid protein import 2) mutant plants, the transcript levels of
Hsc70-4 (one isoform of the Hsc70 family) and CHIP (an E3
ligase) were highly upregulated, which ultimately plays crucial
roles in proteasomal degradation of unimported plastid proteins
(Lee et al., 2009). We also found that, along with those of
Hsc70-4 and CHIP, the transcript level of AtBAG1 (Arabidopsis
thaliana Bcl2-associated athanogene 1) in the ppi2 mutant was
2.38-fold higher than that in the wild-type (Lee et al., 2009). In
mammalian cells, BAG proteins play multiple roles in protein
homeostasis, especially as nucleotide exchange factors of
Hsc70, thereby contributing to protein quality control (Alberti
et al., 2003; Doukhanina et al., 2006). Therefore, in this study,
we examined the role of AtBAG in Hsc70-4-mediated protein
quality control in the cytosol
viability because these proteins accumulate as non-specific
aggregates, which can be toxic to the cell (Lee et al., 2009;
Sroka et al., 2009). Previously, we have shown that in ppi2
(plastid protein import 2) mutant plants, the transcript levels of
Hsc70-4 (one isoform of the Hsc70 family) and CHIP (an E3
ligase) were highly upregulated, which ultimately plays crucial
roles in proteasomal degradation of unimported plastid proteins
(Lee et al., 2009). We also found that, along with those of
Hsc70-4 and CHIP, the transcript level of AtBAG1 (Arabidopsis
thaliana Bcl2-associated athanogene 1) in the ppi2 mutant was
2.38-fold higher than that in the wild-type (Lee et al., 2009). In
mammalian cells, BAG proteins play multiple roles in protein
homeostasis, especially as nucleotide exchange factors of
Hsc70, thereby contributing to protein quality control (Alberti
et al., 2003; Doukhanina et al., 2006). Therefore, in this study,
we examined the role of AtBAG in Hsc70-4-mediated protein
quality control in the cytosol
