교수 및 연구

리서치하이라이트
리서치하이라이트

Blue-conversion of organic dyes produces artifacts in multicolor fluor…

  • 저널명Chem Sci. 2021 Jul 1; 12(25): 8660–8667.
    • 담당교수Sung Ho Ryu
    • 조회34
    • 작성자최고관리자
    • 2021-10-12

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    ■ Abstract

    Multicolor fluorescence imaging is a powerful tool visualizing the spatiotemporal relationship among biomolecules. Here, we report that commonly employed organic dyes exhibit a blue-conversion phenomenon, which can produce severe multicolor image artifacts leading to false-positive colocalization by invading predefined spectral windows, as demonstrated in the case study using EGFR and Tensin2. These multicolor image artifacts become much critical in localization-based superresolution microscopy as the blue-converted dyes are photoactivatable. We provide a practical guideline for the use of organic dyes for multicolor imaging to prevent artifacts derived by blue-conversion.

     

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