Blue-conversion of organic dyes produces artifacts in multicolor fluor…
첨부파일
- 첨부된 파일이 없습니다.
- 담당교수Sung Ho Ryu
- 조회189
- 작성자최고관리자
- 2021-10-12
관련링크
본문
■ Abstract
Multicolor fluorescence imaging is a powerful tool visualizing the spatiotemporal relationship among biomolecules. Here, we report that commonly employed organic dyes exhibit a blue-conversion phenomenon, which can produce severe multicolor image artifacts leading to false-positive colocalization by invading predefined spectral windows, as demonstrated in the case study using EGFR and Tensin2. These multicolor image artifacts become much critical in localization-based superresolution microscopy as the blue-converted dyes are photoactivatable. We provide a practical guideline for the use of organic dyes for multicolor imaging to prevent artifacts derived by blue-conversion.