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포항공과대학교 생명과학과

ENG

대학원

입학안내

입학안내

지도교수 선정

대학원 희망 지도교수 선정

  •  지원자들은 입학원서 연구계획서란에 1, 2, 3 지망 지도교수를 기재하고, 각각 지망 이유를 주십시오.
  •  생명과학과는 4단계 BK21사업(미래인정양성사업- 최우수 대학원 육성 및 연구장학금 지원)을 수행하고 있습니다.

교수진 소개


김민성

LIFE SCIENCES

김민성 부교수

구조생물학

ㆍ연구실
분자인지구조생물학 연구실
ㆍ세부연구분야
단백질-핵산 상호작용, 면역반응 구조분석
ㆍPhone
+82-54-279-2362
ㆍE-mail
cggs@postech.ac.kr
ㆍHomepage
http://mskimlab.postech.ac.kr/

Research introduction

The growth and replication of living eukaryotic cells are regulated by lots of signals from outside and also from inside. Cell receives various signals from other cells by messengers, such as growth factor, which are recognized by cell surface receptors. On the other hands, inside of cell, intensive signals keep firing throughout the multiple networks to process/response the signal from outside and to check/maintain the right metabolism of cells (ex. DNA replication, DNA damage response) and to monitor physiological stress of cells (ex. nutrient depletion)
These inside-cell signaling networks are often composed of sensor, switch, adaptor, transducer and effector, like a sophisticated electric machine.
Among these components, a few numbers of molecular switches control the lots of cellular process. Interestingly, these molecular switches can also integrated the information from sensor and determine the responses.
Any unexpected disruption of these signaling pathway is responsible for human disease.
Dr. Kim is interesting in the biomolecular interactions between the components of signaling pathway molecules to improve our understanding of cellular process and to give a fundamental basis of rational approach to therapeutic intervention.

학부생을 위한 실험실 소개

우리 실험실에서는 서로 다른 단백질간, 단백질과 기질간 또는 단백질과 DNA간의 상호작용을 생화학적, 구조생물학적 방법을 통해 밝힘으로써, 생체내에서 일어나는 일련의 신호전달 활성화 과정을 분자 수준에서 이해 하고자 합니다. 이에 대한 정보를 바탕으로 각종 질병에서 일어나는 비이상적 상호작용을 설명하고, 이를 조절할수 있는 방법 개발하고자 합니다.

현재 진행 하고자 하는 연구 내용으로는
- 박테리아에서 DNA 손상 치료 단백질간의 상호 작용과 활성화 메커니즘 연구
- 식물에서 면역 수용체와 병원균 effector 단백질 간의 상호작용 연구
- 세포내 PRR이 활성화 이후 면역 항상성에 끼치는 영향 연구 등을 진행하고자 합니다.

이에 대해서 학생들이 참여 할수 있는 실험 내용으로는
- 유전자 클론닝 및 발현 벡터 최적화
- E. coli 와 Mammalian protein expression system을 이용한 단백질 발현 및 정제
- Co-ip를 이용한 단백질간의 상호작용 분석, EMSA 등을 수행할 예정입니다.

Research Area

  • Protein engineering
  • Protein/DNA/RNA interaction
  • X-ray crystallography
  • Electron Microscopy
  • Membrane protein crystallography

Major publications

  • Chun Inseop, Kim MS, et al., (2023) Structural basis of DNA binding by the NAC transcription factor ORE1, a master regulator of plant senescence. Plant Communications 4. 100510
  • Kim Jieun, Kim MS, et al., (2021) Crystal structure of human interleukin-2 in complex with TCB2, a new antibody-drug candidate with antitumor activity. OncoImmunology 10, 1899671
  • Lee CH, Kim MS, et al., (2020) Structure-Function Analyses of a Keratin Heterotypic Complex Identify Specific Keratin Regions Involved in Intermediate Filament Assembly. Structure 28, 355-362
  • Kim MS, Wei Yang et al., (2018) Cracking the DNA Code for V(D)J Recombination. Molecular Cell 70, 358-370
  • Tian Lan, Kim MS. et al., (2018) Structure of HIV-1 reverse transcriptase cleaving RNA in an RNA/DNA hybrid. PNAS 115. 507-512
  • Notarangel L.D., Kim MS, et al., (2016) Human RAG mutations: biochemistry and clinical implications. Nat Rev lmmunol 16, 234-246
  • Kim MS, Lapkouski M., et al., (2015) Crystal structure of the V(D)J recombinase RAG1-RAG2. Nature 518, 507-511
  • Lee CH, Kim MS, et al., (2012) Structural basis for heteromeric assembly and perinuclear organization of keratin filaments. NSMB 19, 707-715
  • Kim MS, Saunders A.M., et al., Structure of the protein core of the glypican Dally-like and localization of a region important for hedgehog signaling. PNAS 108, 13112-13117
  • Lim JH, Kim MS, et al., Structural basis for preferential recognition of diaminopimelic acid-type peptidoglycan by a subset of peptidoglycan recognition proteins. JBC 281, 8286-8295
  • Kim MS, Yi MJ, et al., (2005) Biochemical and crystallographic studies reveal a specific interaction between TRAPP subunits Trs33p and Bet3p. Traffic 6, 1183-1195
  • Kim MS, Shin J, et al., (2003) Crystal structures of RbsD leading to the identification of cytoplasmic sugar-binding proteins with a novel folding architecture. JBC 278, 28173-28180
  • Kim MS, Byun M, et al.,(2003) Crystal structure of peptidoglycan recognition protein LB from Drosophila melanogaster. Nat lmmunol 4, 787-793

Education

  • B.S., POSTECH. Korea (1998)
  • M.S., POSTECH. Korea (2000)
  • Ph.D., POSTECH. Korea (2003)

Career

  • 2003-2006 : Postdoctoral Fellow, Dept. of Life Sciences, POSTECH
  • 2006-2012 : Postdoctoral Fellow, Dept. of Biophysics and Biophysical Chemistry, Johns Hopkins University
  • 2012-2014 : Research Fellow, NIDDK, National Institutes of Health
  • 2014-2017 : Staff Scientist, NIDDK, National Institutes of Health
  • 2017-2023 : Assistant Professor, Div. of Integrative Biosciences & Biotechnology (IBB), POSTECH
  • 2023-Present : Associate Professor. Department of Life Sciences, POSTECH

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